SOMERSET BEEKEEPERS ASSOCIATION
  • Home
    • Devon Apicultural Research Group (DARG)
  • About
    • About the SBKA
    • History
    • SBKA Council
    • Who's Who
    • Support Us
    • Donate to SBKA
  • Events
    • All Events
    • Beginners' Courses
    • Advanced Courses
    • Apiary Meetings
    • Honey Shows
    • Lectures & Workshops
  • Members
    • Joining SBKA
    • Getting Started >
      • Education >
        • How To Guides
        • Video Guides
        • Courses and Qualifications
        • Examinations
      • Introduction to Beekeeping
      • Beginners' Courses
      • Life In a Honeybee Colony
      • Frequently Asked Questions
      • Beekeeping Suppliers
    • Useful Links
    • Membership >
      • Benefits
      • Rules and Regs
      • Get Involved
      • Vacancies
      • County Membership
    • Members Area >
      • Members' Blog
      • AGM Info
      • Council Meetings Info
      • Financial Info
      • Lecture recordings
      • Policies
      • Year Book
    • Pests & Diseases >
      • Asian hornet
      • Hygiene
      • Reporting Foulbrood
    • SBKA Newsletters
    • Beekeeping Suppliers
    • Research
  • Divisions
    • Find Nearest Division
    • Burnham >
      • Burnham Beginners Course
      • Burnham Improvers Course
      • Contact Burnham
    • Central >
      • Central Beginners Course
      • Contact Central
    • Exmoor >
      • Exmoor Beginners Course
      • Contact Exmoor
    • Frome >
      • Frome Beginners Course
      • Contact Frome
      • Frome Library
      • Frome Group Apiary
    • Mendip >
      • Mendip Beginners Course
      • Contact Mendip
    • Quantock >
      • Quantock Beginners Course
      • Contact Quantock
    • Somerton >
      • Somerton Beginners Course
      • Contact Somerton
    • South East >
      • South East Beginners Course
      • South East Newsletters
      • Contact South East
    • South West >
      • Contact South West
    • Taunton >
      • Taunton Newsletters
      • Taunton Beginners Course
      • Contact Taunton
    • Wedmore & Cheddar >
      • Wedmore & Cheddar Beginners Course
      • Contact Wedmore & Cheddar
    • Yeovil >
      • Yeovil Beginners Course
      • Yeovil Equipment List
      • Contact Yeovil
  • News
    • Press Releases
    • Image Gallery
    • Resources
  • Contact Us
    • Contact Us
    • Report a Swarm
    • Press & Media
    • Local Honey Search

Members' Blog

SBKA supported EFB research: update

16/11/2021

 
Picture
​Somerset BKA and Bee Diseases Insurance Ltd (BDI) are jointly funding PhD student Hollie Pufal at Newcastle University.
Hollie is exploring the virulent ST2 strain of EFB which is uniquely associated with Somerset and north Dorset.
Hollie and her supervisor Prof Giles Budge will be involved in our Lecture Day on 12 February, 2022.

After completing her first year, she has provided a brief report on her work to date:
"Thank you for your generous funding that has allowed me to pursue research in this exciting area. Over the past 6 months I have been establishing methods to develop a molecular workflow that could be used to generate whole genome sequencing data from infected larvae at outbreak sites, I also tested supers from infected apiaries for the presence of EFB.
I tested 4 locations on each frame for the presence of EFB, as well as capped honey and pollen, using DNA extractions and Realtime PCR. The results showed only a small trace of EFB in a couple of samples both from the frame, pollen and honey.
I plan to sequence using handheld sequencing technology, which is a cheaper and more efficient sequencing platform, so has the potential to be used more readily in the future for assessing EFB outbreaks. The issue with sequencing infected larvae material is that most of the sequence generated will be honeybee DNA, so I originally tested various methods that deplete the bee DNA and enrich the bacterial DNA. Initially I used homogenised larvae mixed with M.plutonius culture and then I moved on to infected larvae from the field. I used qPCR assays to detect both EFB and insect DNA to assess the efficiency of the depletion methods, and I now have a selection of promising methods that I will take forward to sequencing and assess the quality."


Comments are closed.

    Archives

    September 2023
    May 2023
    April 2023
    November 2022
    October 2022
    April 2022
    March 2022
    February 2022
    January 2022
    December 2021
    November 2021
    September 2021
    July 2021
    June 2021
    April 2021
    February 2021
    January 2021
    October 2020
    September 2020
    August 2020
    July 2020
    June 2020
    April 2020
    March 2020
    February 2020
    January 2020
    October 2019
    September 2019
    August 2019
    July 2019
    May 2019
    April 2019
    March 2019
    February 2019
    January 2019
    December 2018
    November 2018
    October 2018
    September 2018
    August 2018
    July 2018
    June 2018
    May 2018
    April 2018
    March 2018
    February 2018
    January 2018
    December 2017
    November 2017
    October 2017
    September 2017
    August 2017

    Categories

    All
    Asian Hornets
    BBKA
    Beekeeper
    Beekeepers
    Bees
    Beginners Beekeepers
    BIBBA
    #EvidenceWeek
    Honey
    Honeybees
    Honey Show
    Local Honey
    Neonicotinoids
    Obituary
    Pollinators
    Presentations
    Save Our Bees
    SBKA Lectures
    SBKA Members
    Swarm
    Swarms
    Training

    RSS Feed

Home

EVENTS

Divisions

Contact

Picture
Somerset Beekeepers Association © 2021     Registered Charity 277803
​Affiliated to the British Beekeepers Association
​Click here to view our Privacy Policy
  • Home
    • Devon Apicultural Research Group (DARG)
  • About
    • About the SBKA
    • History
    • SBKA Council
    • Who's Who
    • Support Us
    • Donate to SBKA
  • Events
    • All Events
    • Beginners' Courses
    • Advanced Courses
    • Apiary Meetings
    • Honey Shows
    • Lectures & Workshops
  • Members
    • Joining SBKA
    • Getting Started >
      • Education >
        • How To Guides
        • Video Guides
        • Courses and Qualifications
        • Examinations
      • Introduction to Beekeeping
      • Beginners' Courses
      • Life In a Honeybee Colony
      • Frequently Asked Questions
      • Beekeeping Suppliers
    • Useful Links
    • Membership >
      • Benefits
      • Rules and Regs
      • Get Involved
      • Vacancies
      • County Membership
    • Members Area >
      • Members' Blog
      • AGM Info
      • Council Meetings Info
      • Financial Info
      • Lecture recordings
      • Policies
      • Year Book
    • Pests & Diseases >
      • Asian hornet
      • Hygiene
      • Reporting Foulbrood
    • SBKA Newsletters
    • Beekeeping Suppliers
    • Research
  • Divisions
    • Find Nearest Division
    • Burnham >
      • Burnham Beginners Course
      • Burnham Improvers Course
      • Contact Burnham
    • Central >
      • Central Beginners Course
      • Contact Central
    • Exmoor >
      • Exmoor Beginners Course
      • Contact Exmoor
    • Frome >
      • Frome Beginners Course
      • Contact Frome
      • Frome Library
      • Frome Group Apiary
    • Mendip >
      • Mendip Beginners Course
      • Contact Mendip
    • Quantock >
      • Quantock Beginners Course
      • Contact Quantock
    • Somerton >
      • Somerton Beginners Course
      • Contact Somerton
    • South East >
      • South East Beginners Course
      • South East Newsletters
      • Contact South East
    • South West >
      • Contact South West
    • Taunton >
      • Taunton Newsletters
      • Taunton Beginners Course
      • Contact Taunton
    • Wedmore & Cheddar >
      • Wedmore & Cheddar Beginners Course
      • Contact Wedmore & Cheddar
    • Yeovil >
      • Yeovil Beginners Course
      • Yeovil Equipment List
      • Contact Yeovil
  • News
    • Press Releases
    • Image Gallery
    • Resources
  • Contact Us
    • Contact Us
    • Report a Swarm
    • Press & Media
    • Local Honey Search